To identify genotypes that maintain pest resistance and incorporate these genotypes into the durable pest resistant breeding program.
Root-knot nematode and phylloxera resistant rootstocks are key to sustainable grapevine production, as Vitis vinifera cultivars are highly susceptible to these pests.
With the development of genotyping-by-sequencing techniques and the application of new genetic mapping programs, tools are now available to combine within the same rootstock root-knot nematode and phylloxera resistance loci from different genetic backgrounds to develop new rootstocks with durable resistance to these soil pests.
The project will evaluate selected rootstocks for release by collecting and analysing data for yield, vigour, fruit and wine quality from four existing CSIRO rootstock field trials established as part of an earlier project (CSP 1304).
Markers linked to root-knot nematode and phylloxera resistance loci from V. cinerea were successfully identified in project CSP 1304. To build upon this research, other Vitis species with resistance will be identified and next generation mapping will be used to identify markers linked to new root-knot nematode and phylloxera resistance loci.
Elite breeding lines containing multiple resistance genes will be produced for developing rootstock genotypes with durable pest resistance. Lastly, in vitro root-knot nematode and phylloxera screens will be developed to identify germplasm material that maintains pest resistance at moderate to high temperatures.
New improved rootstocks with durable resistance to root-knot nematode and phylloxera and other desirable rootstock traits.